1、MODIFIED EPOXY RESIN IMMOBILIZED ENZYME, AND PREPARATION METHOD

The epoxy resin is modified, the polyethyleneimine is added to the modified epoxy resin for the further modifi-cation, and an aldehyde group in the resin and an amino group in the polyethyleneimine are covalently bound to each enzyme, then it is activated by the bifunctional re-agent glutaraldehyde.

2、Upgrading Epoxy Supports for Enzyme Immobilization by Affinity

This article provides a method to upgrade epoxy-functionalized carriers for covalent enzyme immobilization to selective carriers suitable for covalent immobilization of metal affinity-tagged enzymes without the need of preliminary enzyme purification.

3、Modified epoxy resin immobilized enzyme, preparation method therefor

The epoxy resin is modified, the polyethyleneimine is added to the modified epoxy resin for the further modification, and an aldehyde group in the resin and an amino group in the polyethyleneimine are covalently bound to each enzyme, then it is activated by the bifunctional reagent glutaraldehyde.

【中文】改性环氧树脂固定化酶、制备方法及应用 【EN

By applying the technical scheme of the invention, epoxy resin is modified, polyethyleneimine is added into the modified epoxy resin for further modification, aldehyde groups in the resin and amino groups in the polyethyleneimine are covalently bonded to each enzyme, and then the enzyme is activated by a bifunctional reagent of ...

WO/2026/142618 MODIFIED EPOXY RESIN IMMOBILIZED ENZYME, AND PREPARATION

Specifically, the preparation method comprises the following steps: modifying epoxy resin, adding polyethyleneimine to the modified epoxy resin for further modification, and then adding an enzyme to be immobilized and glutaraldehyde for immobilization to obtain a modified epoxy resin immobilized enzyme.

Enzyme Immobilization Procedures

Enzymes covalently immobilized on epoxy functionalized resins are suitable for applications in water or biphasic phase and can be recycled several times until the enzyme is losing activity.

Chitosan

Chitosan-Modified Epoxy Resin for Improving the Performance of an Immobilized Enzyme Carrier Science of Advanced Materials Pub Date : 2018-01-16 , DOI: 10.1166/sam.2018.3151

Site

Here, we developed a convenient strategy to immobilize enzymes on microporous amino resins through transglutaminase-catalyzed bioconjugation. We utilized L-2-haloacid dehalogenase (L-HADST) as a model enzyme.

Chitosan

In this paper, we report a novel in vitro TUDCA preparation technology in which the epimerization of taurochenodeoxycholic acid (TCDCA) to TUDCA is directly catalyzed by...

Modifying the Microenvironment of Epoxy Resin to Improve the Activity

Abstract 7α-Hydroxysteroid dehydrogenase (7α-HSDH) is one of the key enzymes in the catalytic reaction of taurochenodeoxycholic acid (TCDCA). To improve the activity of immobilized 7α-HSDH, the microenvironment of immobilized 7α-HSDH was modified with epoxy resin and ethanediamine (EDA).

In modern biotechnology and bioengineering, enzymes, as highly efficient biological catalysts, are widely utilized in various industrial processes due to their unique catalytic functions. traditional enzyme preparations often face challenges such as poor stability, susceptibility to environmental factors, and difficulties in large-scale application. To overcome these challenges, researchers have developed a novel enzyme immobilization technique: immobilized enzymes using modified epoxy resin. This technology significantly enhances enzyme stability and lifespan by immobilizing enzyme molecules within modified epoxy resin, which possesses excellent mechanical strength and chemical stability. It also provides greater flexibility for enzyme applications.

Modified epoxy resin is a composite material composed of epoxy resin mixed with multiple additives. Compared to traditional epoxy resins, modified epoxy resin exhibits higher mechanical strength, better chemical corrosion resistance, and superior thermal stability. These properties make it an ideal carrier material for enzyme immobilization.

The process of enzyme immobilization typically includes enzyme pretreatment, adsorption, cross-linking, and elution. During the pretreatment stage, enzymes are activated to improve adsorption efficiency on modified epoxy resin. Enzymes are then immobilized via physical or chemical methods. Common immobilization techniques include physical adsorption, covalent binding, and cross-linking. Among these, covalent binding is one of the most widely used methods, as it involves forming specific chemical bonds between enzyme molecules and modified epoxy resin.

The advantages of immobilized enzymes lie in their high stability and reusability. Compared to free enzymes, immobilized enzymes are less affected by external conditions such as temperature, pH, and ion concentration, which enhances the specificity and stability of catalytic reactions. Additionally, immobilized enzymes can be recovered and reused through simple physical methods (e.g., centrifugation), significantly reducing enzyme usage costs.

Immobilized enzymes demonstrate immense potential in practical applications. For example, in the food industry, they can produce high-purity enzyme preparations like lipases, proteases, and amylases, which improve food texture, color, and nutritional value. In pharmaceuticals, immobilized enzymes are used to manufacture antibiotics, antivirals, and anticancer drugs with higher activity and stability. In environmental protection, they degrade organic pollutants and heavy metal ions, mitigating environmental pollution.

Despite its advantages, the technology faces challenges. First, improving the stability and reusability of immobilized enzymes remains a research focus. Second, optimizing process conditions for optimal catalytic performance is critical. Finally, cost reduction and scalability are key factors hindering commercialization.

Looking ahead, ongoing technological advancements will likely expand the applications of modified epoxy resin-immobilized enzymes. Further research and innovation could address existing challenges and drive technological progress. We also anticipate the emergence of new products and technologies based on this approach, offering greater convenience and benefits for humanity.